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Image Search Results
Journal: Gut Microbes
Article Title: Gut microbiota signatures in primary aldosteronism and functional identification of an aldosterone-degrading gut bacterium
doi: 10.1080/19490976.2026.2657047
Figure Lengend Snippet: Validation of R. gnavus -mediated degradation of aldosterone and multiple steroid hormones in germ-free mice. (a) Experimental design. (b) Quantification of R. gnavus colonization in fecal samples by quantitative real-time PCR (qPCR) targeting R. gnavus -specific 16S rRNA gene sequences. (c-d) Fecal (c) and serum (d) aldosterone concentrations in germ-free (GF) mice and GF mice colonized with R. gnavus . (e) mRNA expression levels of aldosterone-responsive genes related to intestinal epithelial ion transport in the distal colon. (f) Fecal concentrations of steroid hormones. 21C, C21 steroid hormones; 19C, C19 steroid hormones; 18C, C18 steroid hormones; 11-DOC, 11-deoxycorticosterone. For (b-c) and (e-f), n = 9 per group. For (d), n = 6 for the PBS group and n = 7 for the R. gnavus group. Data are presented as mean ± SEM. Statistical analysis was performed using unpaired two-tailed t-test. * p < 0.05, ** p < 0.01, *** p < 0.001.
Article Snippet: Following quantification, RNA was reverse transcribed into cDNA using the HiScript III All-in-one
Techniques: Biomarker Discovery, Real-time Polymerase Chain Reaction, Expressing, Two Tailed Test
Journal: Gut Microbes
Article Title: Gut microbiota signatures in primary aldosteronism and functional identification of an aldosterone-degrading gut bacterium
doi: 10.1080/19490976.2026.2657047
Figure Lengend Snippet: Attenuation of exogenous aldosterone-induced physiological alterations by R. gnavus in vivo . (a) Experimental design. (b) Quantification of R. gnavus colonization in fecal samples by qPCR targeting R. gnavus -specific 16S rRNA gene sequences. (c-g) Serum aldosterone concentrations (c), fecal aldosterone concentrations (d), systolic blood pressure (e), serum sodium (f), and serum potassium (g) in antibiotic (ABX)-treated mice receiving VEH + PBS ( n = 5), ALD + PBS ( n = 6), or ALD + RG ( n = 6). VEH, vehicle; ALD, aldosterone; RG, Ruminococcus gnavus ; Serum Na + , serum sodium; Serum K + , serum potassium. For (b-g), data are presented as mean ± SEM. Statistical analysis was performed using One-way ANOVA with Tukey’s multiple comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001.
Article Snippet: Following quantification, RNA was reverse transcribed into cDNA using the HiScript III All-in-one
Techniques: In Vivo